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KMID : 0616120220530020096
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Korean Journal of Pharmacognosy
2022 Volume.53 No. 2 p.96 ~ p.101
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Antioxidant Activity of Ethyl acetate Fraction of Melampyrum roseum Maxim. in Caenorhabditis elegans
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Kim Jun-Hyeong
Park Chang-Bum
Parl Jong-Hyun
Kwon Kang-Mu
Ma Sang-Yong
Oh Suk-Heung
Kim Dae-Keun
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Abstract
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Caenorhabditis elegans model system was used to investigate the antioxidant activity of methanol extract of Melampyrum roseum (Scrophulariaceae). The ethyl acetate soluble fraction of the M. roseum methanol extract showed the best DPPH radical scavenging activity. The ethyl acetate fraction was measured for the activity of superoxide dismutase (SOD), catalase, and oxidative stress tolerance by using C. elegans along with reactive oxygen species (ROS) level. In addition, to confirm that the regulation of the stress response gene is responsible for the increased stress tolerance of C. elegans treated by the ethyl acetate fraction, SOD-3 expression was measured using a transgenic strain. As a result, the ethyl acetate fraction increased SOD and catalase activity, and decreased ROS accumulation in a dose-dependent manner. In addition, the ethyl acetate fraction-treated CF1553 worm showed higher SOD-3::GFP intensity than the control worm.
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KEYWORD
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Melampyrum roseum, Caenorhabditis elegans, Antioxidant activity, Catalase, SOD
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